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Procell Inc human immortalized liver cell line thle 2
( A ) Cell viability of HepG2, <t>Hepa1-6,</t> <t>THLE-2</t> cells assessed under different conditions ( n = 6). ( B ) Cell viability of HepG2 cells pre-protected with Z-VAD-FMK, Nec-1, Dis, Fer-1 and Lip-1 ( n = 6) (** p < 0.01).
Human Immortalized Liver Cell Line Thle 2, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+immortalized+liver+cell+line+thle+2/2+thle/pmc12939784-55-13-23
Average 86 stars, based on 1 article reviews
human immortalized liver cell line thle 2 - by Bioz Stars, 2026-09
86/100 stars

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1) Product Images from "Cucurbitacin B Inhibits Hepatocellular Carcinoma by Inducing Ferroptosis and Activating the cGAS-STING Pathway"

Article Title: Cucurbitacin B Inhibits Hepatocellular Carcinoma by Inducing Ferroptosis and Activating the cGAS-STING Pathway

Journal: Current Issues in Molecular Biology

doi: 10.3390/cimb48020138

( A ) Cell viability of HepG2, Hepa1-6, THLE-2 cells assessed under different conditions ( n = 6). ( B ) Cell viability of HepG2 cells pre-protected with Z-VAD-FMK, Nec-1, Dis, Fer-1 and Lip-1 ( n = 6) (** p < 0.01).
Figure Legend Snippet: ( A ) Cell viability of HepG2, Hepa1-6, THLE-2 cells assessed under different conditions ( n = 6). ( B ) Cell viability of HepG2 cells pre-protected with Z-VAD-FMK, Nec-1, Dis, Fer-1 and Lip-1 ( n = 6) (** p < 0.01).

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Article Title: Cucurbitacin B Inhibits Hepatocellular Carcinoma by Inducing Ferroptosis and Activating the cGAS-STING Pathway
Article Snippet: Cucurbitacin B (CuB) (Yuanye, Shanghai, China, CAS#: 6199-67-3, No: JB245504 , 98% purity); Ferrostatin-1 (Fer-1) (Abcam, Cambridge, UK, No: GR3378683-6); HepG2-specific medium (Procell, Wuhan, China); 0.25% Trypsin-EDTA (Thermo, Waltham, MA, USA); MTT (LabLead, Beijing, China); Dimethyl Sulfoxide(DMSO) (Sigma, St. Louis, MO, USA); Reactive Oxygen Species (ROS) Detection Kit, DAPI Stain, Mitochondrial Membrane Potential Assay Kit (JC-1), Total GSH Detection Kit, Lipid Peroxidation Detection Kit (BODIPY 581/591 C11) (Beyotime, Shanghai, China); Z-VAD-FMK, Necrostatin-1, Disulfiram, Ferrostatin-1, Liproxstatin-1 (MCE, Monmouth Junction, NJ, USA); FeRhoNox-1 (Abmole, Houston, Texas, USA); Malondialdehyde (MDA) Content Assay Kit (AIDISHENG, Yancheng, China); Tris-Glycine-SDS Electrophoresis Buffer, Tris-Glycine Transfer Buffer, SDS-PAGE Gel Preparation Kit (Boster, Wuhan, China); Bicinchoninic Acid (BCA) Protein Assay Kit (UtiBody, Tianjin, China); Mitochondria Isolation Kit, Animal Tissue/Cell Genomic DNA Isolation Kit (Solarbio, Beijing, China); ACSL4/FACL4 Polyclonal antibody, BAX Polyclonal antibody, Caspase 3/P17/P19 Polyclonal antibody, SLC7A11/xCT Polyclonal antibody, GPX4 Polyclonal antibody, BCL2 Polyclonal antibody, TMEM173/STING Polyclonal antibody, CD71 Polyclonal antibody, IRF3 Polyclonal antibody, Phospho-IRF3 (Ser396) Recombinant antibody, TBK1 Polyclonal antibody, Phospho-TBK1 (Ser172) Recombinant antibody, cGAS Polyclonal antibody, IFN-beta Polyclonal antibody (Proteintech, Wuhan, China). .. Human hepatoma cell line HepG2 (CL-0103), mouse-derived hepatoma cell line Hepa1-6 (CL-0105), and human immortalized liver cell line THLE-2 (CL-0833) were obtained from Procell and cultured in accordance with guidance from Procell. .. In brief, HepG2 cells were seeded in a 25 cm 2 cell culture flask (Corning, Corning, NY, USA) and incubated in 5 mL of cell-specific medium (Procell, Wuhan, China).

Article Title: Cucurbitacin B Inhibits Hepatocellular Carcinoma by Inducing Ferroptosis and Activating the cGAS-STING Pathway
Article Snippet: Cucurbitacin B (CuB) (Yuanye, Shanghai, China, CAS#: 6199-67-3, No: JB245504, 98% purity); Ferrostatin-1 (Fer-1) (Abcam, Cambridge, UK, No: GR3378683-6); HepG2specific medium (Procell, Wuhan, China); 0.25% Trypsin-EDTA (Thermo, Waltham, MA, USA); MTT (LabLead, Beijing, China); Dimethyl Sulfoxide(DMSO) (Sigma, St. Louis, MO, USA); Reactive Oxygen Species (ROS) Detection Kit, DAPI Stain, Mitochondrial Membrane Potential Assay Kit (JC-1), Total GSH Detection Kit, Lipid Peroxidation Detection Kit (BODIPY 581/591 C11) (Beyotime, Shanghai, China); Z-VAD-FMK, Necrostatin-1, Disul- https://doi.org/10.3390/cimb48020138 firam, Ferrostatin-1, Liproxstatin-1 (MCE, Monmouth Junction, NJ, USA); FeRhoNox-1 (Abmole, Houston, Texas, USA); Malondialdehyde (MDA) Content Assay Kit (AIDISHENG, Yancheng, China); Tris-Glycine-SDS Electrophoresis Buffer, Tris-Glycine Transfer Buffer, SDS-PAGE Gel Preparation Kit (Boster, Wuhan, China); Bicinchoninic Acid (BCA) Protein Assay Kit (UtiBody, Tianjin, China); Mitochondria Isolation Kit, Animal Tissue/Cell Genomic DNA Isolation Kit (Solarbio, Beijing, China); ACSL4/FACL4 Polyclonal antibody, BAX Polyclonal antibody, Caspase 3/P17/P19 Polyclonal antibody, SLC7A11/xCT Polyclonal antibody, GPX4 Polyclonal antibody, BCL2 Polyclonal antibody, TMEM173/STING Polyclonal antibody, CD71 Polyclonal antibody, IRF3 Polyclonal antibody, Phospho-IRF3 (Ser396) Recombinant antibody, TBK1 Polyclonal antibody, Phospho-TBK1 (Ser172) Recombinant antibody, cGAS Polyclonal antibody, IFN-beta Polyclonal antibody (Proteintech, Wuhan, China). .. Human hepatoma cell line HepG2 (CL-0103), mouse-derived hepatoma cell line Hepa16 (CL-0105), and human immortalized liver cell line THLE-2 (CL-0833) were obtained from Procell and cultured in accordance with guidance from Procell. .. In brief, HepG2 cells were seeded in a 25 cm2 cell culture flask (Corning, Corning, NY, USA) and incubated in 5 mL of cell-specific medium (Procell, Wuhan, China).



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( A ) Cell viability of HepG2, Hepa1-6, THLE-2 cells assessed under different conditions ( n = 6). ( B ) Cell viability of HepG2 cells pre-protected with Z-VAD-FMK, Nec-1, Dis, Fer-1 and Lip-1 ( n = 6) (** p < 0.01).

Journal: Current Issues in Molecular Biology

Article Title: Cucurbitacin B Inhibits Hepatocellular Carcinoma by Inducing Ferroptosis and Activating the cGAS-STING Pathway

doi: 10.3390/cimb48020138

Figure Lengend Snippet: ( A ) Cell viability of HepG2, Hepa1-6, THLE-2 cells assessed under different conditions ( n = 6). ( B ) Cell viability of HepG2 cells pre-protected with Z-VAD-FMK, Nec-1, Dis, Fer-1 and Lip-1 ( n = 6) (** p < 0.01).

Article Snippet: Human hepatoma cell line HepG2 (CL-0103), mouse-derived hepatoma cell line Hepa1-6 (CL-0105), and human immortalized liver cell line THLE-2 (CL-0833) were obtained from Procell and cultured in accordance with guidance from Procell.

Techniques: